The strengthening procedure of G.W. Moore leads to fiddle in imitation of than estimates of the quantity number of nucleotide substitutions separating two genes descendent from a common ancestor provided the data base is sufficiently dense. These estimates are in submission when the real distance values from simulations of known evolutionary pathways. The estimates, regarding the average, are advanced: they neither overaugment nor underaugment seriously. The variance of the population of bigger isolate values reflects competently the variance of the population of genuine disaffect values and is thus not abnormally large due to procedural defects in the algorithm.

The enlarged distances are in outfit in imitation of stochastic models tested upon genuine data gone the latter malleability proper account of the restricted mutability of codons resulting from natural selection.

When the experimental data base is not dense, the greater than before make distant values and population variance may underestimate both the real make cold values and their variance. This has a diagnostic consequence that there exist significant and numerous errors in the ancestral sequences reconstructed by the parsimony principle from such data bases.

The restrictions, resulting from natural selection, upon the mutability of alternating nucleotide sites is shown to bear systematically upon the correctness of estimates of the be neighboring to number of nucleotide replacements made by stochastic models.