I didn't know that people are actually using cysteine to remove trace oxygen ?!
Is it really a general protocol? I thought it is just commonly used for scavenging ROS in cell.
Unsurprisingly, a common amino acid tends to be rather non-toxic to the bugs we want to grow.
Well, lots of broth are just some peptides and amino acids, so cysteine should be there and should be non toxic (but also help bugs grow too ?!!)
Haha XDD
Oh, absolutely. It's fairly common since it's so gentle and non-toxic.
The biggest problem is that it's also slow. We usually add resazuring as a redox indicator at the same time, prior to inoculation. We have to wait for it to go from pink to clear, and this can take a few hours at 37 C, even if we prepare using water boiled under N2/CO2 and keep an anoxic headspace and it's just scavenging the last traces of oxygen.
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Yeah friends do not forget ya!
Be careful at the time of using amino acid.
What do you mean "be careful"?
我终于知道臭屁是什么了。。。哈哈哈
🤣🤣🤣
好尴尬哈哈，下一期活动主题是什么呢，我要密切关注哈哈
哈哈這個可能要延一點
最近在趕進度沒有太多時間審閱文章🙈🙈
嗯我怕错过一天来看好几遍哈哈哈，因为我每次都要想好几天才能想起来适合的东西哈哈。看来我最近可以放松一下了哈哈
在出比賽的時候我在這裏留個言提醒一下吧 XDD
(如果我沒有忘記XD）
感謝支持啊！！！
Sometimes I really admire how you freely use those biological/chemical terms just like breathing. Great information tho!
🤣🤣🤣thanks!!!
Will that be too difficult to be understood?
I can tell you actually try hard to present them in the way eaiser to ubderstand, and you did it.
Thanks for the encouragement!!!
You'd think in micro/molecular bio, we'd be interested in L-cysteine as an amino acid (and we are, when we think of disulfide bridges).
You'd probably be suprised that on a day-to-day basis, we generally think of it as 'that reducing agent we add to anaerobic media to scrub out trace oxygen'. Unsurprisingly, a common amino acid tends to be rather non-toxic to the bugs we want to grow.
I didn't know that people are actually using cysteine to remove trace oxygen ?!
Well, lots of broth are just some peptides and amino acids, so cysteine should be there and should be non toxic (but also help bugs grow too ?!!) Haha XDDIs it really a general protocol? I thought it is just commonly used for scavenging ROS in cell.
Oh, absolutely. It's fairly common since it's so gentle and non-toxic.
The biggest problem is that it's also slow. We usually add resazuring as a redox indicator at the same time, prior to inoculation. We have to wait for it to go from pink to clear, and this can take a few hours at 37 C, even if we prepare using water boiled under N2/CO2 and keep an anoxic headspace and it's just scavenging the last traces of oxygen.
Forgot to add, we generally schedule anaerobic media prep around dinner/pub time for that reason.