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RE: The Homeless Man Who Made Chimeras
You are a great writer and I enjoy reading your posts. Some typos and things you may want to consider:
- pronuclear injection is not only done with injecting sperm but any foreign DNA that the researchers want to get incorporated in the embryo.
- Technically the PEPCK mouse is not a knock out mouse but uses the same technique as you described for your embryonic stem cell chimera. It is a knock in mouse that uses the actin promoter to over-express the enzyme in mice.
Typo: They are "the most accurate in vitro..." This should read in vivo_
Cheers!
Love Genetics!
ian
This is what happens when you rely too much on wikipedia lol! And by you I mean I! The 'in vitro' part for instance is a direct quote. Since the animals are whole and living I guess it means you're right! Fixed.
About the PEPCK mouse, I wrote in the first paragraph ""knock out" mice genes, or put in new ones". But the second time I mention the mouse I leave room for confusion. I added a note.
I fixed the pronuclear injection thing, that was a mistake that I can't blame on anyone else unfortunately. :P
Thanks so much for taking the time to read attentively and comment!
My pleasure and I edited the Wikipedia entry.
I am partly and indirectly responsible for a two-letter change on Wikipedia.
I can now die happy.